By Phyllis R. Brown, Eli Grushka
"Volume 35 examines well timed topics similar to functionality requisites, detection modes, and ancillary innovations for optical detectors in capillary electrophoresis; and more."
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Additional resources for Advances in Chromatography, Volume 35
Provided that the optical characteristics of the reference (I0) and the sample (I) channels are truly identical except for the analyte, compensation should lead to natural cancellation and a more stable baseline. Ideally, a ratio circuit should be used, since Îbc = log (I0/I), where Î is the molar absorptivity, b is the pathlength, and c is the concentration. This can be implemented using log-ratio Page 14 amplifiers. Actual improvements have not been demonstrated, since such devices themselves do not have the necessary resolution or stability.
When all components have been eluted, the measured absorbance returns to its original value. An actual example of axial-beam absorbance detection is shown in Fig. 4 . The predicted positive and negative steps in absorbance are clearly depicted. Several subtle features in this experimental approach should be noted. Even though Fig. 4 does not resemble a typical electropherogram, it contains all the information that is present in an electropherogram. Because the signal has been summed over all components, it is straightforward to show that the first derivative of Fig.
Thermaloptical detectors are ideal for CE because of the slow-flow rates (less cooling) and the small detector volumes (larger temperature increase locally). An example of the impressive detection power associated with photothermal deflection in CE is found in Ref. 25, where attomolar levels of the dabsyl derivatives of amino acids can be monitored. One does have to use a mechanically rigid system to achieve critical alignment of the laser beams. Photothermal detection is also less sensitive in CE compared with LC because of the high-heat capacity and the small coefficient of expansion of water .